Primer Details for TRINITY_DN30849_c0_g1_i1 | |||
|---|---|---|---|
| (AG)8: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCATGCACTAAAAGTTACTACGGT | ACGCTCTCTCTCTCTCTCTTTCT | Start: 26 |
| Tm | 59.067 | 60.055 | End: 258 |
| Size | 24 | 23 | 233 |
| Primer 2 details | |||
| Sequence | CCATGCACTAAAAGTTACTACGGT | ACGCTCTCTCTCTCTCTCTTTC | Start: 26 |
| Tm | 59.067 | 58.729 | End: 258 |
| Size | 24 | 22 | 233 |
| Primer 3 details | |||
| Sequence | TCCATGCACTAAAAGTTACTACGG | ACGCTCTCTCTCTCTCTCTTTCT | Start: 25 |
| Tm | 58.827 | 60.055 | End: 258 |
| Size | 24 | 23 | 234 |
| Primer 4 details | |||
| Sequence | CCATGCACTAAAAGTTACTACGGT | ACGCTCTCTCTCTCTCTCTTT | Start: 26 |
| Tm | 59.067 | 57.65 | End: 258 |
| Size | 24 | 21 | 233 |
| Primer 5 details | |||
| Sequence | TCCATGCACTAAAAGTTACTACGGT | ACGCTCTCTCTCTCTCTCTTTCT | Start: 25 |
| Tm | 60.28 | 60.055 | End: 258 |
| Size | 25 | 23 | 234 |
