Primer Details for TRINITY_DN3057_c0_g1_i1 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTTTGGGGTGGGTCTAGTT | ATGAACCGTTGAGCTAAATTCTGT | Start: 68 |
| Tm | 59.601 | 58.996 | End: 167 |
| Size | 20 | 24 | 100 |
| Primer 2 details | |||
| Sequence | CGTTTGGGGTGGGTCTAGTT | ATGAACCGTTGAGCTAAATTCTGTG | Start: 68 |
| Tm | 59.601 | 60.107 | End: 167 |
| Size | 20 | 25 | 100 |
| Primer 3 details | |||
| Sequence | CCGTTTGGGGTGGGTCTAG | TGAACCGTTGAGCTAAATTCTGTG | Start: 67 |
| Tm | 59.701 | 59.787 | End: 166 |
| Size | 19 | 24 | 100 |
| Primer 4 details | |||
| Sequence | TAAGAAGGAACGGAGGGGAC | ACCGTTGAGCTAAATTCTGTGAG | Start: 47 |
| Tm | 58.435 | 59.003 | End: 163 |
| Size | 20 | 23 | 117 |
| Primer 5 details | |||
| Sequence | TAAGAAGGAACGGAGGGGAC | ACCGTTGAGCTAAATTCTGTGA | Start: 47 |
| Tm | 58.435 | 57.997 | End: 163 |
| Size | 20 | 22 | 117 |
