Primer Details for TRINITY_DN30561_c0_g1_i1 | |||
|---|---|---|---|
| (TAT)5: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGACTTCTAGTTGTAGCATTTGA | GTGTGTGGGGAAGGTGTCTA | Start: 122 |
| Tm | 58.22 | 58.947 | End: 259 |
| Size | 24 | 20 | 138 |
| Primer 2 details | |||
| Sequence | ACGACTTCTAGTTGTAGCATTTGA | GGTGTGTGGGGAAGGTGTC | Start: 122 |
| Tm | 58.22 | 60.228 | End: 260 |
| Size | 24 | 19 | 139 |
| Primer 3 details | |||
| Sequence | ACGACTTCTAGTTGTAGCATTTGA | GTGTGTGGGGAAGGTGTCTAT | Start: 122 |
| Tm | 58.22 | 59.371 | End: 259 |
| Size | 24 | 21 | 138 |
| Primer 4 details | |||
| Sequence | ACGACTTCTAGTTGTAGCATTTGAT | GTGTGTGGGGAAGGTGTCTA | Start: 122 |
| Tm | 58.601 | 58.947 | End: 259 |
| Size | 25 | 20 | 138 |
| Primer 5 details | |||
| Sequence | ACGACTTCTAGTTGTAGCATTTGA | GGTGTGTGGGGAAGGTGTCT | Start: 122 |
| Tm | 58.22 | 61.706 | End: 260 |
| Size | 24 | 20 | 139 |
