ssr_primer
Primer Details for TRINITY_DN30516_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTAAGGCGACCCAAGGCA | TGCAACAAGGCGACAAAAGG | Start: 215 |
| Tm | 60.179 | 59.898 | End: 346 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
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| Sequence | TTTTAAGGCGACCCAAGGCA | ACAAGGCGACAAAAGGGACA | Start: 215 |
| Tm | 60.179 | 60.107 | End: 342 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
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| Sequence | TTTTAAGGCGACCCAAGGCA | GATGAAGGTGCAACAAGGCG | Start: 215 |
| Tm | 60.179 | 60.11 | End: 354 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
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| Sequence | CTTGAGGTGCTTGGGGTCTC | TAGCTGTCTTCAAGGCGCAA | Start: 141 |
| Tm | 60.322 | 59.965 | End: 290 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
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| Sequence | TTTTAAGGCGACCCAAGGCA | GGTGCAACAAGGCGACAAAA | Start: 215 |
| Tm | 60.179 | 60.179 | End: 348 |
| Size | 20 | 20 | 134 |
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