Primer Details for TRINITY_DN30442_c0_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCTGGTTTCCGTTCCTACA | TCAAAGGGAGAATCTTTTTCCTCA | Start: 17 |
| Tm | 59.998 | 58.048 | End: 160 |
| Size | 21 | 24 | 144 |
| Primer 2 details | |||
| Sequence | AGCTCTGGTTTCCGTTCCTAC | TCAAAGGGAGAATCTTTTTCCTCA | Start: 16 |
| Tm | 59.724 | 58.048 | End: 160 |
| Size | 21 | 24 | 145 |
| Primer 3 details | |||
| Sequence | GCTCTGGTTTCCGTTCCTACA | AGGGAGAATCTTTTTCCTCATTCT | Start: 17 |
| Tm | 59.998 | 57.6 | End: 156 |
| Size | 21 | 24 | 140 |
| Primer 4 details | |||
| Sequence | GCTCTGGTTTCCGTTCCTACA | TCAAAGATAAAGGCAACCCAATTT | Start: 17 |
| Tm | 59.998 | 57.568 | End: 195 |
| Size | 21 | 24 | 179 |
| Primer 5 details | |||
| Sequence | GCTCTGGTTTCCGTTCCTACA | TCAAAGGGAGAATCTTTTTCCTCAT | Start: 17 |
| Tm | 59.998 | 58.444 | End: 160 |
| Size | 21 | 25 | 144 |
