Primer Details for TRINITY_DN30362_c0_g1_i1 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTGAAAGTATATAGACAGGGGCGT | GAAAATTTTCCATTGACCAACTTGT | Start: 139 |
| Tm | 60.183 | 57.448 | End: 238 |
| Size | 26 | 25 | 100 |
| Primer 2 details |
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| Sequence | AATTGAAAGTATATAGACAGGGGCG | GAAAATTTTCCATTGACCAACTTGT | Start: 139 |
| Tm | 58.769 | 57.448 | End: 238 |
| Size | 25 | 25 | 100 |
| Primer 3 details |
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| Sequence | AAATTGAAAGTATATAGACAGGGGCG | GAAAATTTTCCATTGACCAACTTGT | Start: 138 |
| Tm | 59.289 | 57.448 | End: 238 |
| Size | 26 | 25 | 101 |
| Primer 4 details |
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| Sequence | GAAATTGAAAGTATATAGACAGGGGCG | GAAAATTTTCCATTGACCAACTTGT | Start: 137 |
| Tm | 60.095 | 57.448 | End: 238 |
| Size | 27 | 25 | 102 |
| Primer 5 details |
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| Sequence | CGAAATTGAAAGTATATAGACAGGGGC | GAAAATTTTCCATTGACCAACTTGT | Start: 136 |
| Tm | 60.095 | 57.448 | End: 238 |
| Size | 27 | 25 | 103 |
