Primer Details for TRINITY_DN30346_c0_g1_i1 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACAATGGTTGAGATTCCCCA | CCACAGTTATTTTCTTCGCAGT | Start: 24 |
| Tm | 57.459 | 57.251 | End: 278 |
| Size | 21 | 22 | 255 |
| Primer 2 details | |||
| Sequence | AGAACAAAGTTGGTGTCACACT | CCACAGTTATTTTCTTCGCAGT | Start: 77 |
| Tm | 58.314 | 57.251 | End: 278 |
| Size | 22 | 22 | 202 |
| Primer 3 details | |||
| Sequence | ACAATGGTTGAGATTCCCCAT | CCACAGTTATTTTCTTCGCAGT | Start: 25 |
| Tm | 57.236 | 57.251 | End: 278 |
| Size | 21 | 22 | 254 |
| Primer 4 details | |||
| Sequence | AACAATGGTTGAGATTCCCCA | ACTTCAAATTCTAAGACTCGGTGGA | Start: 24 |
| Tm | 57.459 | 59.988 | End: 166 |
| Size | 21 | 25 | 143 |
| Primer 5 details | |||
| Sequence | GAAACAATGGTTGAGATTCCCCA | CCACAGTTATTTTCTTCGCAGT | Start: 22 |
| Tm | 59.168 | 57.251 | End: 278 |
| Size | 23 | 22 | 257 |
