Primer Details for TRINITY_DN30307_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAATCTTGAGGGTGCCAATCT | AACTTTGAAAGAGTAGGTAGGACT | Start: 194 |
| Tm | 59.42 | 57.002 | End: 298 |
| Size | 22 | 24 | 105 |
| Primer 2 details | |||
| Sequence | AGAATCTTGAGGGTGCCAATCT | AAACTTTGAAAGAGTAGGTAGGACT | Start: 194 |
| Tm | 59.42 | 57.624 | End: 299 |
| Size | 22 | 25 | 106 |
| Primer 3 details | |||
| Sequence | GAATCTTGAGGGTGCCAATCT | AACTTTGAAAGAGTAGGTAGGACT | Start: 195 |
| Tm | 57.99 | 57.002 | End: 298 |
| Size | 21 | 24 | 104 |
| Primer 4 details | |||
| Sequence | AGAATCTTGAGGGTGCCAATC | AACTTTGAAAGAGTAGGTAGGACT | Start: 194 |
| Tm | 57.99 | 57.002 | End: 298 |
| Size | 21 | 24 | 105 |
| Primer 5 details | |||
| Sequence | GGGTTCCCCTATATATGCTTATTTGA | AGATTGGCACCCTCAAGATTCT | Start: 108 |
| Tm | 58.361 | 59.42 | End: 215 |
| Size | 26 | 22 | 108 |
