Primer Details for TRINITY_DN30186_c0_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAGACCCAATTTCCTTTGGT | CAAAAACTTACCAAATCTTCCATTACA | Start: 203 |
| Tm | 59.549 | 57.243 | End: 333 |
| Size | 22 | 27 | 131 |
| Primer 2 details |
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| Sequence | GGAGACCCAATTTCCTTTGGT | CAAAAACTTACCAAATCTTCCATTACA | Start: 204 |
| Tm | 58.11 | 57.243 | End: 333 |
| Size | 21 | 27 | 130 |
| Primer 3 details |
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| Sequence | AGGAGACCCAATTTCCTTTGG | CAAAAACTTACCAAATCTTCCATTACA | Start: 203 |
| Tm | 57.825 | 57.243 | End: 333 |
| Size | 21 | 27 | 131 |
| Primer 4 details |
|---|
| Sequence | AGGAGACCCAATTTCCTTTGGTA | CAAAAACTTACCAAATCTTCCATTACA | Start: 203 |
| Tm | 59.339 | 57.243 | End: 333 |
| Size | 23 | 27 | 131 |
| Primer 5 details |
|---|
| Sequence | TAGGAGACCCAATTTCCTTTGGT | CAAAAACTTACCAAATCTTCCATTACA | Start: 202 |
| Tm | 59.339 | 57.243 | End: 333 |
| Size | 23 | 27 | 132 |
