Primer Details for TRINITY_DN30142_c0_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTTTTGTAATGAGTGTTGGATAGGA | TGGCTGCAAATATATCTGGCCT | Start: 102 |
| Tm | 59.116 | 59.891 | End: 202 |
| Size | 26 | 22 | 101 |
| Primer 2 details |
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| Sequence | TCCTTTTGTAATGAGTGTTGGATAGG | TGGCTGCAAATATATCTGGCCT | Start: 101 |
| Tm | 59.116 | 59.891 | End: 202 |
| Size | 26 | 22 | 102 |
| Primer 3 details |
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| Sequence | CCTTTTGTAATGAGTGTTGGATAGG | TGGCTGCAAATATATCTGGCCT | Start: 102 |
| Tm | 57.911 | 59.891 | End: 202 |
| Size | 25 | 22 | 101 |
| Primer 4 details |
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| Sequence | ATTTATGTGAGGCTGTGAAGAGG | CGACTAATTGTGATCACTCCATCC | Start: 161 |
| Tm | 58.479 | 59.253 | End: 260 |
| Size | 23 | 24 | 100 |
| Primer 5 details |
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| Sequence | TCCTTTTGTAATGAGTGTTGGATAGGA | TGGCTGCAAATATATCTGGCCT | Start: 101 |
| Tm | 60.257 | 59.891 | End: 202 |
| Size | 27 | 22 | 102 |
