Primer Details for TRINITY_DN29470_c0_g1_i1 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAATTCAGGTTCGTGGATTTGA | GCCGCTGGAAAAGAGATTTC | Start: 410 |
| Tm | 58.859 | 57.449 | End: 633 |
| Size | 23 | 20 | 224 |
| Primer 2 details | |||
| Sequence | TGAGATGCAACACTCTGCTGT | AGTGGAAAAAGGATTGAGATGACA | Start: 82 |
| Tm | 59.929 | 58.366 | End: 312 |
| Size | 21 | 24 | 231 |
| Primer 3 details | |||
| Sequence | TGAGATGCAACACTCTGCTGT | GAGTGGAAAAAGGATTGAGATGACA | Start: 82 |
| Tm | 59.929 | 59.293 | End: 313 |
| Size | 21 | 25 | 232 |
| Primer 4 details | |||
| Sequence | TCAGGTTCGTGGATTTGATTCT | GCCGCTGGAAAAGAGATTTC | Start: 415 |
| Tm | 57.714 | 57.449 | End: 633 |
| Size | 22 | 20 | 219 |
| Primer 5 details | |||
| Sequence | TGCCCCTACTATTTGTTAAACCCT | GCCGCTGGAAAAGAGATTTC | Start: 377 |
| Tm | 59.707 | 57.449 | End: 633 |
| Size | 24 | 20 | 257 |
