ssr_primer
Primer Details for TRINITY_DN290_c0_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAGTGAAATGACGTCGACG | TGAACTCACCGGCCAATCAG | Start: 881 |
| Tm | 59.909 | 60.322 | End: 992 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
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| Sequence | GCAGTGAAATGACGTCGACG | ATGAACTCACCGGCCAATCA | Start: 881 |
| Tm | 59.909 | 59.672 | End: 993 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
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| Sequence | ATGGACCAGCGTTCGAACAT | TTTGTTGTGTTGGCAGCAGC | Start: 262 |
| Tm | 60.036 | 60.46 | End: 541 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
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| Sequence | GCTGCTGCCAACACAACAAA | CACGAGAGATGGAAGAGGGC | Start: 522 |
| Tm | 60.46 | 59.896 | End: 639 |
| Size | 20 | 20 | 118 |
| Primer 5 details |
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| Sequence | ATGGACCAGCGTTCGAACAT | CAGCAGCATGAACCACGAAA | Start: 262 |
| Tm | 60.036 | 59.407 | End: 528 |
| Size | 20 | 20 | 267 |
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