Primer Details for TRINITY_DN28738_c0_g1_i1 | |||
|---|---|---|---|
| (T)10aaaaaaaatgtttaacgtatctactaaatctttgaattattattttttgcatctacaaaagatttaaccttgagttc(T)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCCACTCTTTCAACCAACG | AGATCCAACTTTATCTCGCATTTCT | Start: 29 |
| Tm | 59.689 | 58.647 | End: 295 |
| Size | 20 | 25 | 267 |
| Primer 2 details | |||
| Sequence | TCAGGTGGGGTTAAATGCCC | TCCAACTTTATCTCGCATTTCTTT | Start: 13 |
| Tm | 59.96 | 57.182 | End: 292 |
| Size | 20 | 24 | 280 |
| Primer 3 details | |||
| Sequence | GCCCACTCTTTCAACCAACG | GATCCAACTTTATCTCGCATTTCT | Start: 29 |
| Tm | 59.689 | 57.389 | End: 294 |
| Size | 20 | 24 | 266 |
| Primer 4 details | |||
| Sequence | GCCCACTCTTTCAACCAACG | AGATCCAACTTTATCTCGCATTTC | Start: 29 |
| Tm | 59.689 | 57.389 | End: 295 |
| Size | 20 | 24 | 267 |
| Primer 5 details | |||
| Sequence | TCAGGTGGGGTTAAATGCCC | TCGCATTTCTTTTCTAAAATCCACA | Start: 13 |
| Tm | 59.96 | 58.024 | End: 280 |
| Size | 20 | 25 | 268 |
