Primer Details for TRINITY_DN28691_c0_g1_i1 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTCTCTCACTTCTCGTCA | GGGGTCTATTGCATTCATATCTCC | Start: 3 |
| Tm | 59.201 | 58.811 | End: 245 |
| Size | 20 | 24 | 243 |
| Primer 2 details | |||
| Sequence | TGACGCTCTCTCACTTCTCG | GGGGTCTATTGCATTCATATCTCC | Start: 0 |
| Tm | 59.201 | 58.811 | End: 245 |
| Size | 20 | 24 | 246 |
| Primer 3 details | |||
| Sequence | ACGCTCTCTCACTTCTCGTC | GGGGTCTATTGCATTCATATCTCC | Start: 2 |
| Tm | 59.2 | 58.811 | End: 245 |
| Size | 20 | 24 | 244 |
| Primer 4 details | |||
| Sequence | GACGCTCTCTCACTTCTCGT | GGGGTCTATTGCATTCATATCTCC | Start: 1 |
| Tm | 59.2 | 58.811 | End: 245 |
| Size | 20 | 24 | 245 |
| Primer 5 details | |||
| Sequence | CGCTCTCTCACTTCTCGTCA | GGGGTCTATTGCATTCATATCTCCA | Start: 3 |
| Tm | 59.201 | 60.281 | End: 245 |
| Size | 20 | 25 | 243 |
