Primer Details for TRINITY_DN28639_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTTTCTACTTTTCGGAAAAACCT | TGGAAACATGACCTTGGAGAGA | Start: 160 |
| Tm | 57.599 | 59.021 | End: 261 |
| Size | 25 | 22 | 102 |
| Primer 2 details |
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| Sequence | ACTTTTCTACTTTTCGGAAAAACCT | TGGAAACATGACCTTGGAGAGAG | Start: 160 |
| Tm | 57.599 | 59.992 | End: 261 |
| Size | 25 | 23 | 102 |
| Primer 3 details |
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| Sequence | ACTTTTCTACTTTTCGGAAAAACCT | GGAAACATGACCTTGGAGAGAGA | Start: 160 |
| Tm | 57.599 | 59.738 | End: 260 |
| Size | 25 | 23 | 101 |
| Primer 4 details |
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| Sequence | TGTATCCTCTTTCCTATTTCTTGGA | TGGAAACATGACCTTGGAGAGA | Start: 130 |
| Tm | 57.251 | 59.021 | End: 261 |
| Size | 25 | 22 | 132 |
| Primer 5 details |
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| Sequence | TGTATCCTCTTTCCTATTTCTTGGA | TGGAAACATGACCTTGGAGAGAG | Start: 130 |
| Tm | 57.251 | 59.992 | End: 261 |
| Size | 25 | 23 | 132 |
