Primer Details for TRINITY_DN27821_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCATGAAAAGCTAGCCTATTTTCT | GGCTCAGGGTGGAAACCTTT | Start: 12 |
| Tm | 58.809 | 60.179 | End: 115 |
| Size | 25 | 20 | 104 |
| Primer 2 details | |||
| Sequence | ACCATGAAAAGCTAGCCTATTTTCT | AATGGCTCAGGGTGGAAACC | Start: 12 |
| Tm | 58.809 | 60.252 | End: 118 |
| Size | 25 | 20 | 107 |
| Primer 3 details | |||
| Sequence | ACCATGAAAAGCTAGCCTATTTTC | GGCTCAGGGTGGAAACCTTT | Start: 12 |
| Tm | 57.543 | 60.179 | End: 115 |
| Size | 24 | 20 | 104 |
| Primer 4 details | |||
| Sequence | ACCATGAAAAGCTAGCCTATTTTC | AATGGCTCAGGGTGGAAACC | Start: 12 |
| Tm | 57.543 | 60.252 | End: 118 |
| Size | 24 | 20 | 107 |
| Primer 5 details | |||
| Sequence | ATCTATACCATGAAAAGCTAGCCT | GGGTGGAAACCTTTTCGTGC | Start: 6 |
| Tm | 57.306 | 59.968 | End: 109 |
| Size | 24 | 20 | 104 |
