Primer Details for TRINITY_DN27801_c0_g1_i1 | |||
|---|---|---|---|
| (A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTCCATCTATATACATACCGTGT | ATCATGCGCCTTCTCGAGAC | Start: 83 |
| Tm | 58.749 | 60.249 | End: 190 |
| Size | 25 | 20 | 108 |
| Primer 2 details | |||
| Sequence | ACCGTGTAGTATGGTCACAAATGA | TCATGCATACTCTTCCCTCCA | Start: 101 |
| Tm | 60.021 | 58.237 | End: 222 |
| Size | 24 | 21 | 122 |
| Primer 3 details | |||
| Sequence | ACCGTGTAGTATGGTCACAAATG | TCATGCATACTCTTCCCTCCA | Start: 101 |
| Tm | 58.75 | 58.237 | End: 222 |
| Size | 23 | 21 | 122 |
| Primer 4 details | |||
| Sequence | TGGTCCATCTATATACATACCGTG | ATCATGCGCCTTCTCGAGAC | Start: 83 |
| Tm | 57.231 | 60.249 | End: 190 |
| Size | 24 | 20 | 108 |
| Primer 5 details | |||
| Sequence | GGTCCATCTATATACATACCGTGT | ATCATGCGCCTTCTCGAGAC | Start: 84 |
| Tm | 57.229 | 60.249 | End: 190 |
| Size | 24 | 20 | 107 |
