Primer Details for TRINITY_DN27777_c0_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCTAAGAACAATTACACAAGGCC | CGTGACTGACATGGTATACAACG | Start: 18 |
| Tm | 59.876 | 59.454 | End: 144 |
| Size | 25 | 23 | 127 |
| Primer 2 details |
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| Sequence | ACCTCTAAGAACAATTACACAAGGC | CGTGACTGACATGGTATACAACG | Start: 17 |
| Tm | 59.294 | 59.454 | End: 144 |
| Size | 25 | 23 | 128 |
| Primer 3 details |
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| Sequence | TCTAAGAACAATTACACAAGGCCT | CGTGACTGACATGGTATACAACG | Start: 20 |
| Tm | 58.124 | 59.454 | End: 144 |
| Size | 24 | 23 | 125 |
| Primer 4 details |
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| Sequence | CTCTAAGAACAATTACACAAGGCCT | CGTGACTGACATGGTATACAACG | Start: 19 |
| Tm | 59.06 | 59.454 | End: 144 |
| Size | 25 | 23 | 126 |
| Primer 5 details |
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| Sequence | CCTCTAAGAACAATTACACAAGGCC | ACGTGACTGACATGGTATACAAC | Start: 18 |
| Tm | 59.876 | 58.508 | End: 145 |
| Size | 25 | 23 | 128 |
