Primer Details for TRINITY_DN27727_c0_g1_i1 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTGCACTTCTTGTTCTGCT | TTATTTTGTCTTCCATGCAGAACTCA | Start: 62 |
| Tm | 60.134 | 59.51 | End: 161 |
| Size | 21 | 26 | 100 |
| Primer 2 details | |||
| Sequence | ACCTGCACTTCTTGTTCTGC | TTATTTTGTCTTCCATGCAGAACTCA | Start: 62 |
| Tm | 58.687 | 59.51 | End: 161 |
| Size | 20 | 26 | 100 |
| Primer 3 details | |||
| Sequence | ACCTGCACTTCTTGTTCTGCT | TTGAACAACAAACTGGACATAACT | Start: 62 |
| Tm | 60.134 | 57.232 | End: 217 |
| Size | 21 | 24 | 156 |
| Primer 4 details | |||
| Sequence | ATACCTGCACTTCTTGTTCTGC | ATTTTGTCTTCCATGCAGAACTCA | Start: 60 |
| Tm | 58.923 | 59.171 | End: 159 |
| Size | 22 | 24 | 100 |
| Primer 5 details | |||
| Sequence | ACCTGCACTTCTTGTTCTGCT | TTATTTTGTCTTCCATGCAGAACT | Start: 62 |
| Tm | 60.134 | 57.101 | End: 161 |
| Size | 21 | 24 | 100 |
