ssr_primer
Primer Details for TRINITY_DN27712_c0_g1_i1 |
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| (TCC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCGATCCTCCTCCTCCTC | ACATAAGGGTTTGGGTGGCA | Start: 55 |
| Tm | 59.814 | 59.515 | End: 156 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
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| Sequence | TTTCCGATCCTCCTCCTCCT | ACATAAGGGTTTGGGTGGCA | Start: 54 |
| Tm | 59.363 | 59.515 | End: 156 |
| Size | 20 | 20 | 103 |
| Primer 3 details |
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| Sequence | TTCCGATCCTCCTCCTCCTC | ACATAAGGGTTTGGGTGGCAT | Start: 55 |
| Tm | 59.814 | 59.921 | End: 156 |
| Size | 20 | 21 | 102 |
| Primer 4 details |
|---|
| Sequence | TTCCGATCCTCCTCCTCCTC | AACATAAGGGTTTGGGTGGCA | Start: 55 |
| Tm | 59.814 | 60.133 | End: 157 |
| Size | 20 | 21 | 103 |
| Primer 5 details |
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| Sequence | CGATCCTCCTCCTCCTCCTC | AACATAAGGGTTTGGGTGGCA | Start: 58 |
| Tm | 60.251 | 60.133 | End: 157 |
| Size | 20 | 21 | 100 |
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