Primer Details for TRINITY_DN27648_c0_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTCTCAAACAAATAGGAATCGCT | CGGGCAGATATATGTAGACAAAGA | Start: 85 |
| Tm | 58.827 | 57.974 | End: 203 |
| Size | 25 | 24 | 119 |
| Primer 2 details |
|---|
| Sequence | TCTTCTCAAACAAATAGGAATCGC | CGGGCAGATATATGTAGACAAAGA | Start: 85 |
| Tm | 57.577 | 57.974 | End: 203 |
| Size | 24 | 24 | 119 |
| Primer 3 details |
|---|
| Sequence | CTTCTCAAACAAATAGGAATCGCT | CGGGCAGATATATGTAGACAAAGA | Start: 86 |
| Tm | 57.572 | 57.974 | End: 203 |
| Size | 24 | 24 | 118 |
| Primer 4 details |
|---|
| Sequence | ACAAACACAATCTGTCGATATTTCCA | CGGGCAGATATATGTAGACAAAGA | Start: 59 |
| Tm | 59.57 | 57.974 | End: 203 |
| Size | 26 | 24 | 145 |
| Primer 5 details |
|---|
| Sequence | ACACAATCTGTCGATATTTCCATCT | CGGGCAGATATATGTAGACAAAGA | Start: 63 |
| Tm | 58.414 | 57.974 | End: 203 |
| Size | 25 | 24 | 141 |
