Primer Details for TRINITY_DN27360_c0_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCATCCTTAAGATCAAGCA | AGCTAAAAGATAGCCCAAAAATGCA | Start: 3 |
| Tm | 57.06 | 59.812 | End: 117 |
| Size | 21 | 25 | 115 |
| Primer 2 details | |||
| Sequence | AGCCATCCTTAAGATCAAGCA | GCTAAAAGATAGCCCAAAAATGCA | Start: 3 |
| Tm | 57.06 | 58.581 | End: 116 |
| Size | 21 | 24 | 114 |
| Primer 3 details | |||
| Sequence | AGCCATCCTTAAGATCAAGCA | AGCTAAAAGATAGCCCAAAAATGC | Start: 3 |
| Tm | 57.06 | 58.336 | End: 117 |
| Size | 21 | 24 | 115 |
| Primer 4 details | |||
| Sequence | AGCCATCCTTAAGATCAAGCAT | AGCTAAAAGATAGCCCAAAAATGCA | Start: 3 |
| Tm | 57.55 | 59.812 | End: 117 |
| Size | 22 | 25 | 115 |
| Primer 5 details | |||
| Sequence | AGCCATCCTTAAGATCAAGCAT | GCTAAAAGATAGCCCAAAAATGCA | Start: 3 |
| Tm | 57.55 | 58.581 | End: 116 |
| Size | 22 | 24 | 114 |
