Primer Details for TRINITY_DN26417_c0_g1_i1 |
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| (T)10ctttttttttc(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTAAAAGATTTGAACCATCAACT | AAGCTTCCTTCTTTTTGAACGT | Start: 51 |
| Tm | 57.16 | 57.016 | End: 202 |
| Size | 25 | 22 | 152 |
| Primer 2 details |
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| Sequence | TCAACTTTTTAGATGATGGATTCGT | AAGCTTCCTTCTTTTTGAACGT | Start: 70 |
| Tm | 57.078 | 57.016 | End: 202 |
| Size | 25 | 22 | 133 |
| Primer 3 details |
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| Sequence | AGGTAAAAGATTTGAACCATCAACT | AGAAAGCTTCCTTCTTTTTGAACGT | Start: 51 |
| Tm | 57.16 | 59.874 | End: 205 |
| Size | 25 | 25 | 155 |
| Primer 4 details |
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| Sequence | TCAACTTTTTAGATGATGGATTCGT | AGAAAGCTTCCTTCTTTTTGAACGT | Start: 70 |
| Tm | 57.078 | 59.874 | End: 205 |
| Size | 25 | 25 | 136 |
| Primer 5 details |
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| Sequence | AGGTAAAAGATTTGAACCATCAACT | AAAGCTTCCTTCTTTTTGAACGT | Start: 51 |
| Tm | 57.16 | 57.675 | End: 203 |
| Size | 25 | 23 | 153 |
