Primer Details for TRINITY_DN26077_c0_g1_i1 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGCAACATAGTAGAAAAATTGAAACT | ACTCCTTCACGGAATTGACTGT | Start: 0 |
| Tm | 58.14 | 59.631 | End: 143 |
| Size | 27 | 22 | 144 |
| Primer 2 details |
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| Sequence | AGGCAACATAGTAGAAAAATTGAAACT | CCTTCACGGAATTGACTGTACC | Start: 0 |
| Tm | 58.14 | 58.999 | End: 140 |
| Size | 27 | 22 | 141 |
| Primer 3 details |
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| Sequence | AGGCAACATAGTAGAAAAATTGAAACT | CTCCTTCACGGAATTGACTGT | Start: 0 |
| Tm | 58.14 | 57.954 | End: 142 |
| Size | 27 | 21 | 143 |
| Primer 4 details |
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| Sequence | AGGCAACATAGTAGAAAAATTGAAACT | ACTCCTTCACGGAATTGACTG | Start: 0 |
| Tm | 58.14 | 57.954 | End: 143 |
| Size | 27 | 21 | 144 |
| Primer 5 details |
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| Sequence | AGGCAACATAGTAGAAAAATTGAAACT | TCCTTCACGGAATTGACTGTACC | Start: 0 |
| Tm | 58.14 | 60.307 | End: 141 |
| Size | 27 | 23 | 142 |
