ssr_primer
Primer Details for TRINITY_DN25827_c0_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCTCTGACACCCGATCCA | ATGTGGGGCTAACGAATGCT | Start: 155 |
| Tm | 59.96 | 59.744 | End: 275 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
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| Sequence | ACTCTCTGACACCCGATCCA | GCGGGCAAGAGAAAGTGGTA | Start: 155 |
| Tm | 59.96 | 60.321 | End: 318 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
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| Sequence | GGGGCCGTACTTTGGTGAAA | TGGATCGGGTGTCAGAGAGT | Start: 71 |
| Tm | 60.538 | 59.96 | End: 174 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
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| Sequence | GGGCCGTACTTTGGTGAAAC | TGGATCGGGTGTCAGAGAGT | Start: 72 |
| Tm | 59.405 | 59.96 | End: 174 |
| Size | 20 | 20 | 103 |
| Primer 5 details |
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| Sequence | AAAGAAATCCAGTGGGGCCG | TGGATCGGGTGTCAGAGAGT | Start: 58 |
| Tm | 60.612 | 59.96 | End: 174 |
| Size | 20 | 20 | 117 |
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