Primer Details for TRINITY_DN25793_c0_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAAACACGATCGTTTACCTTTT | TCATTGTTTTCTGTTCTTCCTTTTCA | Start: 104 |
| Tm | 57.262 | 58.164 | End: 207 |
| Size | 23 | 26 | 104 |
| Primer 2 details |
|---|
| Sequence | TTGAAACACGATCGTTTACCTTT | TCATTGTTTTCTGTTCTTCCTTTTCA | Start: 103 |
| Tm | 57.262 | 58.164 | End: 207 |
| Size | 23 | 26 | 105 |
| Primer 3 details |
|---|
| Sequence | TTTGAAACACGATCGTTTACCTT | TCATTGTTTTCTGTTCTTCCTTTTCA | Start: 102 |
| Tm | 57.262 | 58.164 | End: 207 |
| Size | 23 | 26 | 106 |
| Primer 4 details |
|---|
| Sequence | TCGTTTAGATTTGGTGTACAATCGT | TCATTGTTTTCTGTTCTTCCTTTTCA | Start: 51 |
| Tm | 59.072 | 58.164 | End: 207 |
| Size | 25 | 26 | 157 |
| Primer 5 details |
|---|
| Sequence | ATTTGAAACACGATCGTTTACCT | TCATTGTTTTCTGTTCTTCCTTTTCA | Start: 101 |
| Tm | 57.065 | 58.164 | End: 207 |
| Size | 23 | 26 | 107 |
