Primer Details for TRINITY_DN25791_c0_g1_i1 |
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| (A)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTCTCATTTTCCGTCATTGTTCT | ACGACCTTGAACAATCAATGGT | Start: 36 |
| Tm | 59.002 | 58.514 | End: 149 |
| Size | 25 | 22 | 114 |
| Primer 2 details |
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| Sequence | CCATCATCTTTCTTCTCATTTTCCGT | ACGACCTTGAACAATCAATGGT | Start: 26 |
| Tm | 59.905 | 58.514 | End: 149 |
| Size | 26 | 22 | 124 |
| Primer 3 details |
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| Sequence | TCTCTCTCCATCATCTTTCTTCTCA | ACGACCTTGAACAATCAATGGT | Start: 19 |
| Tm | 58.806 | 58.514 | End: 149 |
| Size | 25 | 22 | 131 |
| Primer 4 details |
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| Sequence | TCATCTTTCTTCTCATTTTCCGTCA | ACGACCTTGAACAATCAATGGT | Start: 29 |
| Tm | 58.77 | 58.514 | End: 149 |
| Size | 25 | 22 | 121 |
| Primer 5 details |
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| Sequence | TCTTCTCATTTTCCGTCATTGTTC | ACGACCTTGAACAATCAATGGT | Start: 36 |
| Tm | 57.759 | 58.514 | End: 149 |
| Size | 24 | 22 | 114 |
