Primer Details for TRINITY_DN25771_c0_g1_i1 | |||
|---|---|---|---|
| (AT)9: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACAGCTTTCTTCTCTCTCCT | TCCAAATACCATAAAGAGAGCTCA | Start: 53 |
| Tm | 58.761 | 57.44 | End: 172 |
| Size | 22 | 24 | 120 |
| Primer 2 details | |||
| Sequence | ACACAGCTTTCTTCTCTCTCCT | TCCTTCTCTCAAATTCCAAATACCA | Start: 53 |
| Tm | 58.761 | 58.211 | End: 186 |
| Size | 22 | 25 | 134 |
| Primer 3 details | |||
| Sequence | ACACAGCTTTCTTCTCTCTCCT | TTCCAAATACCATAAAGAGAGCTCA | Start: 53 |
| Tm | 58.761 | 58.042 | End: 173 |
| Size | 22 | 25 | 121 |
| Primer 4 details | |||
| Sequence | ACACAGCTTTCTTCTCTCTCCTT | TCCAAATACCATAAAGAGAGCTCA | Start: 53 |
| Tm | 59.354 | 57.44 | End: 172 |
| Size | 23 | 24 | 120 |
| Primer 5 details | |||
| Sequence | AACACAGCTTTCTTCTCTCTCCT | TCCAAATACCATAAAGAGAGCTCA | Start: 52 |
| Tm | 59.354 | 57.44 | End: 172 |
| Size | 23 | 24 | 121 |
