Primer Details for TRINITY_DN25743_c0_g1_i1 |
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| (AG)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAAAAAGAGAGAGAGAGTAAAGGT | ACCCTTCAACAAAAGCGTGAG | Start: 91 |
| Tm | 59.215 | 59.324 | End: 203 |
| Size | 26 | 21 | 113 |
| Primer 2 details |
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| Sequence | AGGAAAAAGAGAGAGAGAGTAAAGGT | ACCCTTCAACAAAAGCGTGA | Start: 91 |
| Tm | 59.215 | 58.242 | End: 203 |
| Size | 26 | 20 | 113 |
| Primer 3 details |
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| Sequence | GGAAAAAGAGAGAGAGAGTAAAGGT | ACCCTTCAACAAAAGCGTGAG | Start: 92 |
| Tm | 58 | 59.324 | End: 203 |
| Size | 25 | 21 | 112 |
| Primer 4 details |
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| Sequence | AGGAAAAAGAGAGAGAGAGTAAAGGT | CCCTTCAACAAAAGCGTGAGA | Start: 91 |
| Tm | 59.215 | 59.053 | End: 202 |
| Size | 26 | 21 | 112 |
| Primer 5 details |
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| Sequence | GGAAAAAGAGAGAGAGAGTAAAGGT | ACCCTTCAACAAAAGCGTGA | Start: 92 |
| Tm | 58 | 58.242 | End: 203 |
| Size | 25 | 20 | 112 |
