ssr_primer
Primer Details for TRINITY_DN25742_c0_g1_i1 |
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| (AGA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGATGTCGTTGGTGTGGCT | ATGTTCACCTTACGCCCCTG | Start: 20 |
| Tm | 59.891 | 60.035 | End: 250 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
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| Sequence | AGGCCACGATGAATGGGATG | ATGTTCACCTTACGCCCCTG | Start: 142 |
| Tm | 60.179 | 60.035 | End: 250 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
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| Sequence | AAGATGTCGTTGGTGTGGCT | TCTTGTCGCGTCTCTCCATG | Start: 20 |
| Tm | 59.891 | 59.829 | End: 185 |
| Size | 20 | 20 | 166 |
| Primer 4 details |
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| Sequence | TGTGGCTAGAAGCAGCTCTG | ATGTTCACCTTACGCCCCTG | Start: 33 |
| Tm | 59.751 | 60.035 | End: 250 |
| Size | 20 | 20 | 218 |
| Primer 5 details |
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| Sequence | GTGTGGCTAGAAGCAGCTCT | ATGTTCACCTTACGCCCCTG | Start: 32 |
| Tm | 59.751 | 60.035 | End: 250 |
| Size | 20 | 20 | 219 |
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