Primer Details for TRINITY_DN25733_c0_g1_i1 | |||
|---|---|---|---|
| (A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTTATGATAGCCACCCAAAGC | TGTTTAAGAAATTTTGTGGGTCGT | Start: 33 |
| Tm | 59.377 | 57.79 | End: 210 |
| Size | 22 | 24 | 178 |
| Primer 2 details | |||
| Sequence | GCATAATTTGGCCCAATGAGGA | TGTTTAAGAAATTTTGTGGGTCGT | Start: 53 |
| Tm | 59.3 | 57.79 | End: 210 |
| Size | 22 | 24 | 158 |
| Primer 3 details | |||
| Sequence | AGCATAATTTGGCCCAATGAGG | TGTTTAAGAAATTTTGTGGGTCGT | Start: 52 |
| Tm | 59.298 | 57.79 | End: 210 |
| Size | 22 | 24 | 159 |
| Primer 4 details | |||
| Sequence | ATGATAGCCACCCAAAGCAT | TGTTTAAGAAATTTTGTGGGTCGT | Start: 37 |
| Tm | 57.238 | 57.79 | End: 210 |
| Size | 20 | 24 | 174 |
| Primer 5 details | |||
| Sequence | GGTTATGATAGCCACCCAAAGC | TTGTTTAAGAAATTTTGTGGGTCGT | Start: 33 |
| Tm | 59.377 | 58.368 | End: 211 |
| Size | 22 | 25 | 179 |
