ssr_primer
Primer Details for TRINITY_DN25689_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGACCTTGTGATTTGAAGGA | TACGGATCGGGGAAGAGGTT | Start: 54 |
| Tm | 57.515 | 60.033 | End: 195 |
| Size | 21 | 20 | 142 |
| Primer 2 details |
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| Sequence | ACGACCTTGTGATTTGAAGGA | TTACGGATCGGGGAAGAGGT | Start: 54 |
| Tm | 57.515 | 60.033 | End: 196 |
| Size | 21 | 20 | 143 |
| Primer 3 details |
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| Sequence | ACGACCTTGTGATTTGAAGGA | GCATGTTACGGATCGGGGAA | Start: 54 |
| Tm | 57.515 | 60.179 | End: 201 |
| Size | 21 | 20 | 148 |
| Primer 4 details |
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| Sequence | ACCATCTCTTTTCACCATAGGCT | GCATGTTACGGATCGGGGAA | Start: 100 |
| Tm | 59.475 | 60.179 | End: 201 |
| Size | 23 | 20 | 102 |
| Primer 5 details |
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| Sequence | ACGACCTTGTGATTTGAAGGA | ATGCATGTTACGGATCGGGG | Start: 54 |
| Tm | 57.515 | 60.25 | End: 203 |
| Size | 21 | 20 | 150 |
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