Primer Details for TRINITY_DN25577_c0_g1_i1 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTCCCTAGAAAGTGTTGGG | TGGAATGGATTCTACAATTGGTTCA | Start: 91 |
| Tm | 59.297 | 58.748 | End: 227 |
| Size | 21 | 25 | 137 |
| Primer 2 details |
|---|
| Sequence | GGTCCCTAGAAAGTGTTGGGT | TGGAATGGATTCTACAATTGGTTCA | Start: 92 |
| Tm | 59.296 | 58.748 | End: 227 |
| Size | 21 | 25 | 136 |
| Primer 3 details |
|---|
| Sequence | TCGTGATTGGAAAGAAGAAGAAGA | TCTCTGTTTCATCACGAGCTGT | Start: 18 |
| Tm | 58.146 | 59.703 | End: 149 |
| Size | 24 | 22 | 132 |
| Primer 4 details |
|---|
| Sequence | GGTCCCTAGAAAGTGTTGGGTT | TGGAATGGATTCTACAATTGGTTCA | Start: 92 |
| Tm | 59.892 | 58.748 | End: 227 |
| Size | 22 | 25 | 136 |
| Primer 5 details |
|---|
| Sequence | TGGTCCCTAGAAAGTGTTGGG | TGGAATGGATTCTACAATTGGTTCAA | Start: 91 |
| Tm | 59.297 | 59.276 | End: 227 |
| Size | 21 | 26 | 137 |
