Primer Details for TRINITY_DN25486_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCTTGCAACAAGAAACACACA | AGTATCATTCTCTTTACACAGAGGA | Start: 67 |
| Tm | 59.746 | 57.037 | End: 193 |
| Size | 23 | 25 | 127 |
| Primer 2 details |
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| Sequence | TGTCTTGCAACAAGAAACACACA | TGGCAAGTAAACCAATAGTATCATTCT | Start: 67 |
| Tm | 59.746 | 58.947 | End: 209 |
| Size | 23 | 27 | 143 |
| Primer 3 details |
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| Sequence | TGTCTTGCAACAAGAAACACACA | TGGCAAGTAAACCAATAGTATCATTC | Start: 67 |
| Tm | 59.746 | 57.779 | End: 209 |
| Size | 23 | 26 | 143 |
| Primer 4 details |
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| Sequence | TGTCTTGCAACAAGAAACACACA | GGCAAGTAAACCAATAGTATCATTCT | Start: 67 |
| Tm | 59.746 | 57.548 | End: 208 |
| Size | 23 | 26 | 142 |
| Primer 5 details |
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| Sequence | GTCTTGCAACAAGAAACACACA | AGTATCATTCTCTTTACACAGAGGA | Start: 68 |
| Tm | 58.168 | 57.037 | End: 193 |
| Size | 22 | 25 | 126 |
