Primer Details for TRINITY_DN25397_c0_g1_i1 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCATCACTGTATTTTCAGCAAAGA | TGGGTCCTCCAATACGAAAAA | Start: 35 |
| Tm | 59.525 | 57.208 | End: 255 |
| Size | 25 | 21 | 221 |
| Primer 2 details | |||
| Sequence | GCATCACTGTATTTTCAGCAAAGA | TGGGTCCTCCAATACGAAAAA | Start: 36 |
| Tm | 58.297 | 57.208 | End: 255 |
| Size | 24 | 21 | 220 |
| Primer 3 details | |||
| Sequence | AGCATCACTGTATTTTCAGCAAAG | TGGGTCCTCCAATACGAAAAA | Start: 35 |
| Tm | 58.293 | 57.208 | End: 255 |
| Size | 24 | 21 | 221 |
| Primer 4 details | |||
| Sequence | AGCATCACTGTATTTTCAGCAAA | TGGGTCCTCCAATACGAAAAA | Start: 35 |
| Tm | 57.289 | 57.208 | End: 255 |
| Size | 23 | 21 | 221 |
| Primer 5 details | |||
| Sequence | AAGCATCACTGTATTTTCAGCAA | TGGGTCCTCCAATACGAAAAA | Start: 34 |
| Tm | 57.289 | 57.208 | End: 255 |
| Size | 23 | 21 | 222 |
