Primer Details for TRINITY_DN25275_c0_g1_i1 | |||
|---|---|---|---|
| (A)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAGTTGCATAAGGAGCCC | TTCCCATCTGCATAAGGCTATT | Start: 25 |
| Tm | 59.543 | 57.27 | End: 208 |
| Size | 20 | 22 | 184 |
| Primer 2 details | |||
| Sequence | GCAAGTTGCATAAGGAGCCC | GCCTTCTCCCTAGAAAATACTGACA | Start: 25 |
| Tm | 59.543 | 60.105 | End: 142 |
| Size | 20 | 25 | 118 |
| Primer 3 details | |||
| Sequence | GCAAGTTGCATAAGGAGCCC | AGCCTTCTCCCTAGAAAATACTGAC | Start: 25 |
| Tm | 59.543 | 59.87 | End: 143 |
| Size | 20 | 25 | 119 |
| Primer 4 details | |||
| Sequence | GCAAGTTGCATAAGGAGCCC | AGCCTTCTCCCTAGAAAATACTGA | Start: 25 |
| Tm | 59.543 | 58.719 | End: 143 |
| Size | 20 | 24 | 119 |
| Primer 5 details | |||
| Sequence | GCAAGTTGCATAAGGAGCCC | GCCTTCTCCCTAGAAAATACTGAC | Start: 25 |
| Tm | 59.543 | 58.639 | End: 142 |
| Size | 20 | 24 | 118 |
