Primer Details for TRINITY_DN25117_c0_g1_i1 | |||
|---|---|---|---|
| (A)16: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGGAGTTTGTCAATAGTTCACA | TTGTATGCTTCTGGCTTACCA | Start: 16 |
| Tm | 58.076 | 57.288 | End: 149 |
| Size | 24 | 21 | 134 |
| Primer 2 details | |||
| Sequence | TCAGGAGTTTGTCAATAGTTCACA | ACAGCTAAATTGTATGCTTCTGGC | Start: 16 |
| Tm | 58.076 | 59.903 | End: 158 |
| Size | 24 | 24 | 143 |
| Primer 3 details | |||
| Sequence | TCAGGAGTTTGTCAATAGTTCACAA | TTGTATGCTTCTGGCTTACCA | Start: 16 |
| Tm | 58.647 | 57.288 | End: 149 |
| Size | 25 | 21 | 134 |
| Primer 4 details | |||
| Sequence | TTCAGGAGTTTGTCAATAGTTCACA | TTGTATGCTTCTGGCTTACCA | Start: 15 |
| Tm | 58.647 | 57.288 | End: 149 |
| Size | 25 | 21 | 135 |
| Primer 5 details | |||
| Sequence | TCATTTGGTAAGCCAGAAGCA | ACCTTCTTCTCTCTGTCTACAAAT | Start: 124 |
| Tm | 57.847 | 57.073 | End: 245 |
| Size | 21 | 24 | 122 |
