ssr_primer
Primer Details for TRINITY_DN25106_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTTTTCACAATGGCCAATGGA | ATGGACCAAGTAGGGAGGGG | Start: 42 |
| Tm | 59.609 | 60.328 | End: 189 |
| Size | 23 | 20 | 148 |
| Primer 2 details |
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| Sequence | ACTTTTTCACAATGGCCAATGGA | GGACCAAGTAGGGAGGGGTA | Start: 42 |
| Tm | 59.609 | 59.659 | End: 187 |
| Size | 23 | 20 | 146 |
| Primer 3 details |
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| Sequence | AGGAAGACAACTTTGAGGTCAGT | ATGGACCAAGTAGGGAGGGG | Start: 74 |
| Tm | 59.544 | 60.328 | End: 189 |
| Size | 23 | 20 | 116 |
| Primer 4 details |
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| Sequence | AGGAAGACAACTTTGAGGTCAGT | GGACCAAGTAGGGAGGGGTA | Start: 74 |
| Tm | 59.544 | 59.659 | End: 187 |
| Size | 23 | 20 | 114 |
| Primer 5 details |
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| Sequence | ACTTTTTCACAATGGCCAATGG | ATGGACCAAGTAGGGAGGGG | Start: 42 |
| Tm | 58.258 | 60.328 | End: 189 |
| Size | 22 | 20 | 148 |
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