Primer Details for TRINITY_DN24938_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCTCTAAAACCATCGGCCT | TGGTTTCTTCATTTTGGTTCATTCA | Start: 35 |
| Tm | 59.79 | 58.117 | End: 167 |
| Size | 21 | 25 | 133 |
| Primer 2 details |
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| Sequence | TCGGTCCTCTAAAACCATCGG | TGGTTTCTTCATTTTGGTTCATTCA | Start: 32 |
| Tm | 59.521 | 58.117 | End: 167 |
| Size | 21 | 25 | 136 |
| Primer 3 details |
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| Sequence | TCCTCTAAAACCATCGGCCT | TGGTTTCTTCATTTTGGTTCATTCA | Start: 36 |
| Tm | 58.42 | 58.117 | End: 167 |
| Size | 20 | 25 | 132 |
| Primer 4 details |
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| Sequence | GTCCTCTAAAACCATCGGCCT | TGGTTTCTTCATTTTGGTTCATTCAA | Start: 35 |
| Tm | 59.79 | 58.666 | End: 167 |
| Size | 21 | 26 | 133 |
| Primer 5 details |
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| Sequence | GTCCTCTAAAACCATCGGCCT | TTGGTTTCTTCATTTTGGTTCATTCA | Start: 35 |
| Tm | 59.79 | 58.666 | End: 168 |
| Size | 21 | 26 | 134 |
