Primer Details for TRINITY_DN24814_c0_g1_i1 | |||
|---|---|---|---|
| (T)16: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAATTTTAAATCACTGCTCCA | AGCCCAAAGTGTATAAACGGA | Start: 122 |
| Tm | 57.534 | 57.293 | End: 239 |
| Size | 23 | 21 | 118 |
| Primer 2 details | |||
| Sequence | GTTGCAATTTTAAATCACTGCTCCA | AGCCCAAAGTGTATAAACGGA | Start: 120 |
| Tm | 59.304 | 57.293 | End: 239 |
| Size | 25 | 21 | 120 |
| Primer 3 details | |||
| Sequence | TGTTGCAATTTTAAATCACTGCTCC | AGCCCAAAGTGTATAAACGGA | Start: 119 |
| Tm | 59.304 | 57.293 | End: 239 |
| Size | 25 | 21 | 121 |
| Primer 4 details | |||
| Sequence | TGCAATTTTAAATCACTGCTCCA | AGCCCAAAGTGTATAAACGGAA | Start: 122 |
| Tm | 57.534 | 57.976 | End: 239 |
| Size | 23 | 22 | 118 |
| Primer 5 details | |||
| Sequence | TGCAATTTTAAATCACTGCTCCAA | AGCCCAAAGTGTATAAACGGA | Start: 122 |
| Tm | 58.148 | 57.293 | End: 239 |
| Size | 24 | 21 | 118 |
