Primer Details for TRINITY_DN24442_c0_g1_i1 |
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| (TA)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGAGGAATTCTTAGTTTAATGCTTT | ACACACCCAACCAATTTAATGCA | Start: 16 |
| Tm | 57.438 | 59.611 | End: 178 |
| Size | 26 | 23 | 163 |
| Primer 2 details |
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| Sequence | ATCGAGGAATTCTTAGTTTAATGCTT | ACACACCCAACCAATTTAATGCA | Start: 15 |
| Tm | 57.261 | 59.611 | End: 178 |
| Size | 26 | 23 | 164 |
| Primer 3 details |
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| Sequence | AATCGAGGAATTCTTAGTTTAATGCT | ACACACCCAACCAATTTAATGCA | Start: 14 |
| Tm | 57.261 | 59.611 | End: 178 |
| Size | 26 | 23 | 165 |
| Primer 4 details |
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| Sequence | GAATCGAGGAATTCTTAGTTTAATGCT | ACACACCCAACCAATTTAATGCA | Start: 13 |
| Tm | 58.168 | 59.611 | End: 178 |
| Size | 27 | 23 | 166 |
| Primer 5 details |
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| Sequence | AGAATCGAGGAATTCTTAGTTTAATGC | ACACACCCAACCAATTTAATGCA | Start: 12 |
| Tm | 58.168 | 59.611 | End: 178 |
| Size | 27 | 23 | 167 |
