Primer Details for TRINITY_DN24383_c0_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTGAGCTTTCAAAGTGTGT | TTGCTAAATAAGTTCGGTTAATTGAAC | Start: 76 |
| Tm | 57.995 | 57.385 | End: 179 |
| Size | 21 | 27 | 104 |
| Primer 2 details |
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| Sequence | ACCTGAGCTTTCAAAGTGTGTT | TTGCTAAATAAGTTCGGTTAATTGAAC | Start: 76 |
| Tm | 58.644 | 57.385 | End: 179 |
| Size | 22 | 27 | 104 |
| Primer 3 details |
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| Sequence | TTATGGTAGTTCTACATTGAAAATGGA | ACTCTCCGACTTGATGAACAACA | Start: 40 |
| Tm | 57.033 | 59.93 | End: 154 |
| Size | 27 | 23 | 115 |
| Primer 4 details |
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| Sequence | TATAAATACCTGAGCTTTCAAAGTGTG | GTTCGGTTAATTGAACTCTCCGAC | Start: 69 |
| Tm | 58.054 | 59.909 | End: 168 |
| Size | 27 | 24 | 100 |
| Primer 5 details |
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| Sequence | TATAAATACCTGAGCTTTCAAAGTGTG | GTTCGGTTAATTGAACTCTCCGA | Start: 69 |
| Tm | 58.054 | 58.76 | End: 168 |
| Size | 27 | 23 | 100 |
