Primer Details for TRINITY_DN2402_c0_g1_i1 | |||
|---|---|---|---|
| (T)14: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATCAACATGGACTAGTCTTCG | AGGGTTGATCCATTACTTAACCA | Start: 31 |
| Tm | 59.446 | 57.21 | End: 152 |
| Size | 23 | 23 | 122 |
| Primer 2 details | |||
| Sequence | GCATCAACATGGACTAGTCTTCG | GGGTTGATCCATTACTTAACCATGT | Start: 31 |
| Tm | 59.446 | 59.108 | End: 151 |
| Size | 23 | 25 | 121 |
| Primer 3 details | |||
| Sequence | GCATCAACATGGACTAGTCTTCG | AGCATAAAGGGTTGATCCATTACT | Start: 31 |
| Tm | 59.446 | 57.982 | End: 159 |
| Size | 23 | 24 | 129 |
| Primer 4 details | |||
| Sequence | GCATCAACATGGACTAGTCTTCG | GGGTTGATCCATTACTTAACCATGTC | Start: 31 |
| Tm | 59.446 | 59.961 | End: 151 |
| Size | 23 | 26 | 121 |
| Primer 5 details | |||
| Sequence | GTGCATCAACATGGACTAGTCT | AGGGTTGATCCATTACTTAACCA | Start: 29 |
| Tm | 58.133 | 57.21 | End: 152 |
| Size | 22 | 23 | 124 |
