Primer Details for TRINITY_DN23955_c0_g1_i1 |
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| (AT)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGGGCCACAACAATCCAA | CTCATTCAAATCACTCATTTTGCTT | Start: 159 |
| Tm | 59.813 | 57.115 | End: 265 |
| Size | 20 | 25 | 107 |
| Primer 2 details |
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| Sequence | TTGAGGGCCACAACAATCCA | CTCATTCAAATCACTCATTTTGCTT | Start: 158 |
| Tm | 59.813 | 57.115 | End: 265 |
| Size | 20 | 25 | 108 |
| Primer 3 details |
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| Sequence | TGAGGGCCACAACAATCCAA | TCATTCAAATCACTCATTTTGCTTTCA | Start: 159 |
| Tm | 59.813 | 59.073 | End: 264 |
| Size | 20 | 27 | 106 |
| Primer 4 details |
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| Sequence | TTGAGGGCCACAACAATCCA | TCATTCAAATCACTCATTTTGCTTTCA | Start: 158 |
| Tm | 59.813 | 59.073 | End: 264 |
| Size | 20 | 27 | 107 |
| Primer 5 details |
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| Sequence | TGAGGGCCACAACAATCCAA | CATTCAAATCACTCATTTTGCTTTCA | Start: 159 |
| Tm | 59.813 | 57.921 | End: 263 |
| Size | 20 | 26 | 105 |
