Primer Details for TRINITY_DN23943_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCGTCGTGTTCTTGATGA | TGGCAAGAGATTGAAGATGAACA | Start: 88 |
| Tm | 57.195 | 58.345 | End: 245 |
| Size | 20 | 23 | 158 |
| Primer 2 details | |||
| Sequence | TTCCGTCGTGTTCTTGATGA | TGGCAAGAGATTGAAGATGAACAA | Start: 88 |
| Tm | 57.195 | 58.928 | End: 245 |
| Size | 20 | 24 | 158 |
| Primer 3 details | |||
| Sequence | TTCCGTCGTGTTCTTGATGA | AGAGATTGAAGATGAACAATGGCAC | Start: 88 |
| Tm | 57.195 | 59.875 | End: 240 |
| Size | 20 | 25 | 153 |
| Primer 4 details | |||
| Sequence | TCCGTCGTGTTCTTGATGATT | TGGCAAGAGATTGAAGATGAACA | Start: 89 |
| Tm | 57.687 | 58.345 | End: 245 |
| Size | 21 | 23 | 157 |
| Primer 5 details | |||
| Sequence | TTCCGTCGTGTTCTTGATGAT | TGGCAAGAGATTGAAGATGAACA | Start: 88 |
| Tm | 57.687 | 58.345 | End: 245 |
| Size | 21 | 23 | 158 |
