Primer Details for TRINITY_DN23783_c0_g1_i1 | |||
|---|---|---|---|
| (A)19: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCAATGCGGTGGACAAAC | TGATATGTAGTTGGAAGGTCATCC | Start: 133 |
| Tm | 60.038 | 57.825 | End: 258 |
| Size | 20 | 24 | 126 |
| Primer 2 details | |||
| Sequence | ATGCAATGCGGTGGACAAAC | ATGATATGTAGTTGGAAGGTCATCC | Start: 133 |
| Tm | 60.038 | 58.226 | End: 259 |
| Size | 20 | 25 | 127 |
| Primer 3 details | |||
| Sequence | AATGCGGTGGACAAACCCA | TGATATGTAGTTGGAAGGTCATCC | Start: 137 |
| Tm | 60.153 | 57.825 | End: 258 |
| Size | 19 | 24 | 122 |
| Primer 4 details | |||
| Sequence | TATGCAATGCGGTGGACAAAC | TGATATGTAGTTGGAAGGTCATCC | Start: 132 |
| Tm | 59.799 | 57.825 | End: 258 |
| Size | 21 | 24 | 127 |
| Primer 5 details | |||
| Sequence | ATGCAATGCGGTGGACAAAC | AGTGTATGATATGTAGTTGGAAGGTCA | Start: 133 |
| Tm | 60.038 | 59.655 | End: 264 |
| Size | 20 | 27 | 132 |
