Primer Details for TRINITY_DN23521_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGAGGGAGGCAAGAGTTC | AAAAACAGTAATTAAATGGTCGTCAAA | Start: 81 |
| Tm | 59.085 | 57.195 | End: 182 |
| Size | 20 | 27 | 102 |
| Primer 2 details |
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| Sequence | AAAACCATAATGGAGGGAGGCA | TTAAATGGTCGTCAAATATTGGAAGA | Start: 72 |
| Tm | 59.685 | 57.431 | End: 171 |
| Size | 22 | 26 | 100 |
| Primer 3 details |
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| Sequence | ATGGAGGGAGGCAAGAGTTC | AAAACAGTAATTAAATGGTCGTCAAAT | Start: 81 |
| Tm | 59.085 | 57.025 | End: 181 |
| Size | 20 | 27 | 101 |
| Primer 4 details |
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| Sequence | GGAGGGAGGCAAGAGTTCG | AAAAACAGTAATTAAATGGTCGTCAAA | Start: 83 |
| Tm | 59.781 | 57.195 | End: 182 |
| Size | 19 | 27 | 100 |
| Primer 5 details |
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| Sequence | GGAGGGAGGCAAGAGTTCGA | AAAAACAGTAATTAAATGGTCGTCAAA | Start: 83 |
| Tm | 61.258 | 57.195 | End: 182 |
| Size | 20 | 27 | 100 |
