Primer Details for TRINITY_DN23244_c0_g1_i1 |
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| (AT)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGTATTTAAGGAGTTCTTGTGTTT | CCACCCAATAGTACAACCACAC | Start: 52 |
| Tm | 57.252 | 58.921 | End: 223 |
| Size | 26 | 22 | 172 |
| Primer 2 details |
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| Sequence | ACAGTATTTAAGGAGTTCTTGTGTTT | CCACCCAATAGTACAACCACACT | Start: 52 |
| Tm | 57.252 | 60.245 | End: 223 |
| Size | 26 | 23 | 172 |
| Primer 3 details |
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| Sequence | ACAGTATTTAAGGAGTTCTTGTGTTT | CCACCCAATAGTACAACCACA | Start: 52 |
| Tm | 57.252 | 57.573 | End: 223 |
| Size | 26 | 21 | 172 |
| Primer 4 details |
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| Sequence | ACAGTATTTAAGGAGTTCTTGTGTTT | CACCCAATAGTACAACCACACT | Start: 52 |
| Tm | 57.252 | 57.984 | End: 222 |
| Size | 26 | 22 | 171 |
| Primer 5 details |
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| Sequence | ACAGTATTTAAGGAGTTCTTGTGTTTA | CCACCCAATAGTACAACCACAC | Start: 52 |
| Tm | 57.159 | 58.921 | End: 223 |
| Size | 27 | 22 | 172 |
