ssr_primer
Primer Details for TRINITY_DN22875_c0_g1_i1 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCAATCCAAACAATGCCT | TGTTGCTCGCTTTTCTTCGT | Start: 105 |
| Tm | 58.672 | 58.704 | End: 293 |
| Size | 21 | 20 | 189 |
| Primer 2 details |
|---|
| Sequence | GCTGGAAAATGTTTGGCAAGC | ACCTGAAGTATCGAGCCAAGT | Start: 228 |
| Tm | 59.468 | 58.82 | End: 370 |
| Size | 21 | 21 | 143 |
| Primer 3 details |
|---|
| Sequence | ACACCAATCCAAACAATGCCT | GCTTGCCAAACATTTTCCAGC | Start: 105 |
| Tm | 58.672 | 59.468 | End: 248 |
| Size | 21 | 21 | 144 |
| Primer 4 details |
|---|
| Sequence | AACACCAATCCAAACAATGCCT | TGTTGCTCGCTTTTCTTCGT | Start: 104 |
| Tm | 59.295 | 58.704 | End: 293 |
| Size | 22 | 20 | 190 |
| Primer 5 details |
|---|
| Sequence | GCTGGAAAATGTTTGGCAAGC | ACCTGAAGTATCGAGCCAAGTT | Start: 228 |
| Tm | 59.468 | 59.432 | End: 370 |
| Size | 21 | 22 | 143 |
(ATT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCAATCCAAACAATGCCT | TGTTGCTCGCTTTTCTTCGT | Start: 105 |
| Tm | 58.672 | 58.704 | End: 293 |
| Size | 21 | 20 | 189 |
| Primer 2 details |
|---|
| Sequence | GCTGGAAAATGTTTGGCAAGC | ACCTGAAGTATCGAGCCAAGT | Start: 228 |
| Tm | 59.468 | 58.82 | End: 370 |
| Size | 21 | 21 | 143 |
| Primer 3 details |
|---|
| Sequence | ACACCAATCCAAACAATGCCT | GCTTGCCAAACATTTTCCAGC | Start: 105 |
| Tm | 58.672 | 59.468 | End: 248 |
| Size | 21 | 21 | 144 |
| Primer 4 details |
|---|
| Sequence | AACACCAATCCAAACAATGCCT | TGTTGCTCGCTTTTCTTCGT | Start: 104 |
| Tm | 59.295 | 58.704 | End: 293 |
| Size | 22 | 20 | 190 |
| Primer 5 details |
|---|
| Sequence | GCTGGAAAATGTTTGGCAAGC | ACCTGAAGTATCGAGCCAAGTT | Start: 228 |
| Tm | 59.468 | 59.432 | End: 370 |
| Size | 21 | 22 | 143 |
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