Primer Details for TRINITY_DN22833_c0_g1_i1 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCAATGGATAACTAAGGCTCCA | AAAAATGAGTAGAAGGTTGAAAGCA | Start: 41 |
| Tm | 59.54 | 57.653 | End: 140 |
| Size | 23 | 25 | 100 |
| Primer 2 details | |||
| Sequence | GAGCAATGGATAACTAAGGCTCC | AAAATGAGTAGAAGGTTGAAAGCA | Start: 40 |
| Tm | 58.931 | 57.04 | End: 139 |
| Size | 23 | 24 | 100 |
| Primer 3 details | |||
| Sequence | AGAGCAATGGATAACTAAGGCTCC | AAATGAGTAGAAGGTTGAAAGCAA | Start: 39 |
| Tm | 60.202 | 57.04 | End: 138 |
| Size | 24 | 24 | 100 |
| Primer 4 details | |||
| Sequence | AGAGCAATGGATAACTAAGGCTCC | AAAATGAGTAGAAGGTTGAAAGCA | Start: 39 |
| Tm | 60.202 | 57.04 | End: 139 |
| Size | 24 | 24 | 101 |
| Primer 5 details | |||
| Sequence | AGCAATGGATAACTAAGGCTCCA | AAAAATGAGTAGAAGGTTGAAAGCAA | Start: 41 |
| Tm | 59.54 | 58.219 | End: 140 |
| Size | 23 | 26 | 100 |
